Estimation of the in vivo recombination rate for a plant RNA virus.
نویسندگان
چکیده
Phylogenomic evidence suggested that recombination is an important evolutionary force for potyviruses, one of the larger families of plant RNA viruses. However, mixed-genotype potyvirus infections are marked by low levels of cellular coinfection, precluding template switching and recombination events between virus genotypes during genomic RNA replication. To reconcile these conflicting observations, we evaluated the in vivo recombination rate (rg) of Tobacco etch virus (TEV; genus Potyvirus, family Potyviridae) by coinfecting plants with pairs of genotypes marked with engineered restriction sites as neutral markers. The recombination rate was then estimated using two different approaches: (i) a classical approach that assumed recombination between marked genotypes can occur in the whole virus population, rendering an estimate of rg = 7.762 × 10(-8) recombination events per nucleotide site per generation, and (ii) an alternative method that assumed recombination between marked genotypes can occur only in coinfected cells, rendering a much higher estimate of rg = 3.427 × 10(-5) recombination events per nucleotide site per generation. This last estimate is similar to the TEV mutation rate, suggesting that recombination should be at least as important as point mutation in creating variability. Finally, we compared our mutation and recombination rate estimates to those reported for animal RNA viruses. Our analysis suggested that high recombination rates may be an unavoidable consequence of selection for fast replication at the cost of low fidelity.
منابع مشابه
Evolutionary features of 8K (KDa) silencing suppressor protein of Potato mop-top virus
The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...
متن کاملThe Major Sources of Genetic Differentiation Among Apricot Latent Virus (ApLV) Isolates
Background and Aims: Apricot latent virus (ApLV) is a species within Foveavirus genus (Betaflexiviridae family, Tymovirales order). Phylogenetic analyses using different ORFs nucleotide sequences divided most ApLV isolates into two clusters. However, there is little data about the sources of genetic differentiation among ApLV isolates. Materials and Methods: Partial coat protein (CP) sequences...
متن کاملDetecting past recombination events in Potato virus Y genomic sequences using statistical methods
Molecular evolution of RNA viruses: Plant RNA viruses evolve more rapidly than the DNA of their plant hosts due to a higher mutation rate. Mutational processes include nucleotide substitution that replaces one nucleotide by another, and insertions and/or deletions that result in sequence length changes. In addition to mutation, inter-strain recombination can produce new mosaic strains9. It is i...
متن کاملAssociation of Tomato Leaf Curl New Delhi Virus, Betasatellite, and Alphasatellite with Mosaic Disease of Spine Gourd (Momordica dioica Roxb. Willd) in India
Background: Spine gourd (Momordica dioica Roxb. Willd) is one of the important cucurbitaceous crops grown across the world for vegetable and medicinal purposes. Diseases caused by the DNA viruses are becoming the limiting factors for the production of spine gourd reducing its potential yield. For the commercial cultivation of the spine gourd, propagation material used by most o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of general virology
دوره 95 Pt 3 شماره
صفحات -
تاریخ انتشار 2014